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“Many people praise and acknowledge the healing power of plants, but few people actually take action to prevent their extension by planting and conserving them for future generations.” (Ernest Rukangira )

Wednesday, 3 December 2014

Study of Genetic Transformation of Medicinal Plants, Withania Somnifera (L.) Dunal By Agrobacterium Tumefaciens (MTCC-431

Genetic transformation of Withania somnifera (L.) Dunal was carried out using Agrobacterium tumefaciens strain MTCC-
431. Explants such as leaf, root and seeds were used for genetic transformation. MS basal medium supplemented with different combination of hormones tried for regeneration of plants from different explants. Twenty four hours infection period for seedling was found suitable for genetic transformation. Soaking of seed prior to infection period of 72 hours produces
80% germination which was comparatively higher than that of observed at 24 and 48 hours. Different concentration of growth hormones GA out of 2 mg/ml produces 60% germination in 7 days when seed are infected with 24 hours. Sucrose 3 concentration of 15 mg respond better compared to 9 and 12 mg supplemented in MS growth medium. Enzymes localization on native PAGE for perioxidase, catalase, amylse and superoxide dismutase reveals appearance of new isozymes variants in infected seedling. HPLC analysis of gibbrellic acid treated and infected seedling versus infected seedling without showed different chromatogram. Present study was undertaken to standardize protocol for genetic transformation in a important medicinal plant will be useful for the enhancement of increased production of bioactive metabolites.
Key words: Withania somnifera, Agrobacterium tumefaciens, HPLC, Isoenzyme

http://www.ajebs.com/vol3%283%29/16.pdf
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